Direct Quantification of Damaged Nucleotides in Oligonucleotides Using an Aerolysin Single Molecule Interface

cost benefits of rehabilitation after acute coronary syndrome in iran; using an epidemiological model

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Digital direct detection of microRNAs using single molecule arrays

MicroRNAs (miRNAs) are involved in many biological pathways, and detecting miRNAs accurately is critical for diagnosing a variety of diseases including cancer. However, most current methods for miRNA detection require lengthy sample preparation and amplification steps that can bias the results. In addition, lack of specificity and reproducibility give rise to various challenges in detection of ...

Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays.

We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show t...

Conformationally Constrained Nucleosides, Nucleotides and Oligonucleotides

ARTICLES ON SINGLE-MOLECULE PHYSICS AND CHEMISTRY Direct measurements of memory effects in single-molecule kinetics

Statistics and correlations of single-molecule sequences of modulated reactions are explicitly evaluated in the stochastic rate representation. The memory function, introduced through the Gaussian approximation of the stochastic rate expression, characterizes the correlation in single-molecule rate processes in a formalism similar to the stochastic line shape theory. Within this formalism, the ...